By Audet I, Laforest JP, Martineau GP, Matte JJ. Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada. The aim of the present study was to determine the effects of dietary supplements of vitamins on vitamin status, libido, and semen characteristics in young boars under normal and intensive semen collection.

Brine samples were collected at intervals during the production process and analyzed to determine the total plate count and the numbers of lactic acid bacteria, pseudomonads, Brochothrix thermosphacta, and Enterobacteriaceae.

Listeria monocytogenes numbers in the brine were determined using a PCR with primers for the hemolysin gene in combination with a most probable numbers determination. Maximum numbers of bacteria (log CFU/ml) recovered from the brine after 2.5 h of recirculation were as follows: total plate count, 4.50; lactic acid bacteria, 2.99; pseudomonads, 3.95; B. thermosphacta, 2.79; and enterics, 3.01.

There was an increase in the number of L. monocytogenes in the recirculating brine with time, reaching a maximum of 2.34 log CFU/100 ml after 2.5 h of moisture-enhanced pork production. Thus, recirculating brines can harbor large populations of spoilage bacteria and L. monocytogenes and are an important source of contamination for moisture-enhanced pork.

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Source: PubMed - National Center for Biotechnology Information - March 2004